Betalains from Amaranthus tricolor L
نویسندگان
چکیده
Betalains in Amaranthus tricolor leaf were identified by means of reversed phase high-performance liquid chromatography (HPLC) and Liquid chromatography-Mass spectrometry (LC-MS). In addition to the known compound red-violet amaranthin, two yellow pigments were detected in Amaranthus tricolor. A novel betaxanthin, methyl derivative of arginine betaxanthin was identified on the basis of UV-Vis spectra and mass spectrometric characteristics, as well as by comparison with literature data, which to the best of our knowledge has not been reported in Amaranthus tricolor. Amaranthin was the major betacyanin pigment present in Amaranthus tricolor although methylated arginine betaxanthin, a novel betaxanthin hitherto not observed naturally and betalamic acid were also present. The identified compounds were then evaluated for alpha-amylase inhibitory potential using Bernfeld method. Amaranthin and betaxanthin did not show alpha-amylase inhibitory activity. Betalamic acid displayed significant alpha-amylase inhibitory activity compared to that of a reference standard, acarbose Keyword: Amaranthus tricolor; Betalains; Amaranthin; Methylated Arginine Betaxanthin; Betalamic Acid; Liquid Chromatography-Mass Spectrometry
منابع مشابه
Identification of a Catalase-Phenol Oxidase in Betalain Biosynthesis in Red Amaranth (Amaranthus cruentus)
Betalains are a group of nitrogen-containing pigments that color plants in most families of Caryophyllales. Their biosynthesis has long been proposed to begin with hydroxylation of L-tyrosine to L-DOPA through monophenolase activity of tyrosinase, but biochemical evidence in vivo remains lacking. Here we report that a Group 4 catalase, catalase-phenol oxidase (named as AcCATPO), was identified,...
متن کاملTranscriptome Analysis by Illumina High-Throughout Paired-End Sequencing Reveals the Complexity of Differential Gene Expression during In Vitro Plantlet Growth and Flowering in Amaranthus tricolor L.
Amaranthus tricolor L. is a C4 plant, which is consumed as a major leafy vegetable in some tropical countries. Under conditions of high temperature and short daylight, Am. tricolor readily bolts and blooms, degrading leaf quality. A preliminary in vitro flowering study demonstrated that the flowering control pathway in Am. tricolor may differ from that of Arabidopsis. Nevertheless, no transcrip...
متن کاملBetacyanins and phenolic compounds from Amaranthus spinosus L. and Boerhavia erecta L.
Stem bark extracts of Boerhavia erecta L. (erect spiderling) and Amaranthus spinosus L. (spiny amaranth), two wild growing weed plants used in traditional African medicine, were characterized with respect to their phenolic profile including the betalains. While the main betalains in A. spinosus were identified as amaranthine and isoamaranthine, the major betacyanins in B. erecta were betanin, i...
متن کاملاثر تنش شوری و افزایش دیاکسید کربن بر تجمع پرولین، کربوهیدرات و سایر صفات مورفوفیزیولوژیک گل زلف عروس (Amaranthus tricolor L)
High carbon dioxide (CO2) concentration in atmosphere has direct effects on plants activities. In order to investigate the effect of increased CO2 concentration from 380 to 700 and 1050 mg/L on some chemical and physiological characteristics of Amaranthus tricolor under three salinity treatments (0, 150, and 300 mM NaCl), an experiment was conducted in Research Greenhouse of Ferdowsi University...
متن کاملAnti-nociceptive and anti-inflammatory activity of hydroalcoholic extract of leaves of Amaranthus tricolor L
Amaranthus tricolor Linn. (FamilyAmaranthaceae) popularly known as Joseph’s coat in English. The plant has been used as hepatoprotective and hypoglycemic in Indian system of traditional medicine. In present study the hydroalcoholic extract of leaves of Amaranthus tricolor L. (HAEAT) 100, 200 and 400 mg/kg body weight was studied for anti-nociceptive and anti-inflammatory activities in various a...
متن کامل